PENGARUH PEMBERIAN SERBUK TEMBAKAU (Nicotiana tobacum) DENGAN DOSIS BERBEDA TERHADAP MORTALITAS IKAN NILA (Oreochromis niloticus) SKALA LABORATORIUM DI DESA GRINTING KECAMATAN BULAKAMBA BREBES

ROSADI, IDHAM ALI (2024) PENGARUH PEMBERIAN SERBUK TEMBAKAU (Nicotiana tobacum) DENGAN DOSIS BERBEDA TERHADAP MORTALITAS IKAN NILA (Oreochromis niloticus) SKALA LABORATORIUM DI DESA GRINTING KECAMATAN BULAKAMBA BREBES. Skripsi thesis, Fakultas Perikanan dan Ilmu Kelautan : Budidaya Perairan.

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Abstract

Idham Ali Rosadi 3220600004, Effect of Application of Tobacco Powder (Nicotiana tobacum) at Different Doses on Mortality of Tilapia Fish (Oreochromis niloticus) on Laboratory Scale in Desa Grinting, Kecamatan Bulakamba, Kabupaten Brebes. (Supervisor : Nurjanah, Sri Mulatsih Tilapia is a pest in shrimp ponds that needs to be eradicated because it is a competitor for food, space and oxygen so it can disrupt the life of cultivated shrimp. Tobacco leaves (Nicotiana tabacum) contain nicotine which can be used as a botanical pesticide. Nicotine is an organic chemical compound that is toxic and can be used to kill pests and is a neurotoxin that reacts very quickly. Nicotine alkaloids, nicotine sulfate and other nicotine constituents can be used as contact poisons, fumigants and stomach poisons. The use of tobacco powder as a poison for pond pests is still not widely used compared to tea seed extract. This research aims to determine the effect of application of tobacco powder on the mortality of tilapia as pests in ponds. The materials used in the research were low quality tobacco powder and tilapia fish measuring 7 - 9 cm. The research was carried out using an experimental method with a completely randomized design with treatments A (0 ppm or control), B (10 ppm), C (20 ppm) and D (30 ppm) with three replications. Ten tilapia fish were kept in an aerated bucket containing 20 liters of brackish water (salinity 20 ppt). Tobacco powder in each treatment was prepared by soaking it in water in a glass container for five minutes to extract the toxic suspension. Visual observations of the fish were carried out after ten minutes of soaking with tobacco powder applied to each treatment to determine symptoms of tilapia poisoning. Fish mortality data was calculated at 1 and 24 hours after application. Data analysis used analysis of variance (One Way Anova) to compare the effect of treatments A (0 ppm), B (10 ppm), C (20 ppm) and D (30 ppm) on tilapia mortality 1 and 24 hours after application. The results of the study showed that visually the tilapia began to experience changes in behavior after 10 minutes of tobacco powder application. Observations of tilapia mortality after one hour of tobacco powder application in treatments A (0 ppm or control), B (10 ppm), C (20 ppm) and D (30 ppm)showed figures of 0%, 90%, 100% and 100% respectively.The results of statistical tests showed significant differences between treatment and control. The best treatment for tilapia (Oreochromis niloticus) mortality was treatment C (20 ppm) and D (30 ppm) followed by B (10 ppm) while treatment A (0 ppm) had no effect on tilapia (Oreochromis niloticus) mortality. The results of measuring water quality parameters such as temperature, pH, Dissolved Oxygen (DO), and salt content (salinity) show a suitable range for tilapia (Oreochromis niloticus). Keywords: Pests, Tilapia (Oreochromis niloticus), Pesticides, Tobacco (Nicotiana tabacum)

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